Coding

Part:BBa_K3978039:Design

Designed by: Minqi Hu   Group: iGEM21_HiZJU-China   (2021-10-21)


neget-ClpX


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 700
    Illegal EcoRI site found at 1045
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 700
    Illegal EcoRI site found at 1045
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 700
    Illegal EcoRI site found at 1045
    Illegal BglII site found at 879
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 700
    Illegal EcoRI site found at 1045
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 700
    Illegal EcoRI site found at 1045
    Illegal AgeI site found at 361
    Illegal AgeI site found at 1099
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization is needed if this protein is used in other bacteria.


Source

This part is from E.coli K12.

References